Table 2. Morphometric analysis of tight junction ultrastructure.

Tight junction ultrastructure was analyzed using freeze-fracture electron microcopy (*p < 0.05 versus uninduced (Dox+) cells). The analysis between the groups Dox+ and Dox− was blinded

	WT#15	WT#15	D65N#16	D65N#16
	Dox+	Dox−	Dox+	Dox−
(n)	(7)	(10)	(13)	(12)
Number of horizontal strands	4.14 ± 0.46	3.50 ± 0.22	3.75 ± 0.25	4.31 ± 0.41
Vertical meshwork depth (nm)	204.3 ± 42.8	196.0 ± 33.0	229.6 ± 37.3	228.1 ± 33.5
Breaks per μm strand length	0.06 ± 0.06	0.14 ± 0.10	0.02 ± 0.02	0.09 ± 0.05
Particle-type/continuous strands %	29/71	70/30*	17/83	46/54*