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Proliferation of PBMCs isolated from mice immunized via the intranasal route. PBMCs from individual mice were purified and pooled within each group (4 mice per group); 1 × 105 PBMCs for each group were incubated with rAgE6 for 7 days. PMBC proliferation was measured using the thymidine incorporation assay. Results are presented as counts per minute (CPM). Bars represent means of triplicate values ± SEMs. ***, P <0.001.
