FIG 7.

W3110 gadX::kanR shows sensitivity to chloramphenicol. Growth curves were determined in LBK–100 mM PIPES (pH 7.0) with 5 mM benzoate and 8 μg/ml chloramphenicol, as for Fig. 6. At 16 h, the gadX::kanR knockout mutant and the marA::kanR strain both grew significantly less than strain W3110. The cell density of strain G5-2 showed no difference from those of G5-2 gadX⁺ or G5-2 gadX⁺ rob⁺ (Friedman test and post hoc Conover pairwise comparisons with Holm-Bonferroni-adjusted P values).