TABLE 3.
MICs of benzoate-evolved strains for the antibiotics chloramphenicol and tetracycline with or without salicylatea
| Strain | MIC (μg/ml)b |
|||
|---|---|---|---|---|
| Chloramphenicol |
Tetracycline |
|||
| No inducer | 2 mM salicylate | No inducer | 2 mM salicylate | |
| W3110 | 6 | 16 | 1 | 4 |
| W3110 marA::kanR | 6 | 8 | 1.5 | 4 |
| A1-1 | 6 | 8 | 1.5 | 2 |
| A5-1 | 6 | 8 | 2.5 | 1 |
| C3-1 | 6 | 8 | 1 | 3 |
| E1-1 | 6 | 16 | 1 | 4 |
| G5-1 | 7 | 12 | 1 | 2 |
| G5-2 | 4 | 8 | 1 | 2 |
a
Strains were cultured in LBK–100 mM MOPS (pH 7.0) with or without 2 mM salicylate for 22 h. Positive for growth was defined as an OD600 of ≥0.05 at 22 h. The results shown represent one of three trials overall.
b
Median values from 8 replicates are shown.