TABLE 2.
Relative mRNA levels of virulence genes in strains MGAS2221, M100 (covSΔ133TTCTT137), and M122 (rocAC605A) under in vitro and in vivo conditions
| Condition | Gene | Relative mRNA level or fold change in expressiona |
||
|---|---|---|---|---|
| MGAS2221 | M122 | M100 | ||
| Exponential growth phase | hasA | 1 | 197 | 111 |
| spyCEP | 1 | 252 | 141 | |
| sse | 1 | 55 | 54 | |
| speB | 1 | −9 | −63 | |
| sda1 | 1 | 33 | 26 | |
| scpA | 1 | 29 | 21 | |
| slo | 1 | 241 | 142 | |
| nga | 1 | 301 | 198 | |
| sagA | 1 | 1.8 | 0.86 | |
| Stationary phase | hasA | 1 | 1 | 5 |
| spyCEP | 1 | 6 | 8 | |
| sse | 1 | 1 | 6 | |
| speB | 1 | 0.50 | −1,366 | |
| sda1 | 1 | 5.8 | 4 | |
| scpA | 1 | 2 | 6 | |
| slo | 1 | 6 | 18 | |
| nga | 1 | 5 | 15 | |
| sagA | 1 | 6.4 | 1.4 | |
| In vivo | hasA | 1 | 24 | 64 |
| spyCEP | 1 | 3 | 6 | |
| sse | 1 | 4 | 8 | |
| speB | 1 | 1 | −76 | |
| sda1 | 1 | 1.8 | 2.1 | |
| scpA | 1 | 2 | 3.9 | |
| slo | 1 | 1.5 | 1.6 | |
| nga | 1 | 1.9 | 1.9 | |
| sagA | 1 | 3.5 | −2.6 | |
| In vivo and exponential phaseb | hasA | 2 | 0.22 | 0.97 |
| spyCEP | 40 | 0.46 | 1.84 | |
| sse | 3 | 0.22 | 0.49 | |
| speB | 155 | 1,458 | 122 | |
| sda1 | 3.2 | 0.20 | 0.22 | |
| scpA | 1.8 | 0.13 | 0.33 | |
| slo | 16 | 0.25 | 0.36 | |
| nga | 15 | 0.09 | 0.15 | |
| sagA | 66 | 125 | 30 | |
a
The real-time RT-PCR data in Fig. 2 and for additional genes were first normalized to those for the internal gyrA control and then to those for MGAS2221.
b
The normalized in vivo data for each gene of each strain were divided by those for the same strain at the exponential growth phase.