FIG 2.

β-(1,3)-Glucan exposure and chitin levels are increased in cho1Δ/Δ, kre5Δ/Δ, and caspofungin-treated wild-type (WT) cells compared to those in untreated wild-type cells. (A) Cells were stained with anti-β-(1,3)-glucan primary antibodies, Cy3-labeled secondary antibodies, and calcofluor white (CFW). Three biological replicates were carried out for all experiments. (B) Graph of the relative mean fluorescent intensity of each strain, as quantified by Image J. These data reveal that kre5Δ/Δ mutant cells, caspofungin (cas)-treated cells, and cho1Δ/Δ mutant cells have greater exposure of β-(1,3)-glucan and higher levels of chitin than the wild type (*, P < 0.0001; ø, P < 0.0026).