TABLE 1.
Bacterial strains and plasmids used in the study
| Bacterial strain or genotype or plasmid | Description | Source or reference |
|---|---|---|
| Strains | ||
| AIEC | Serotype O83:H1 | 48 |
| WT EPEC 2348/69 | O127:H6 isolate | 49 |
| ΔescN | Deletion of escN from WT EPEC | 49 |
| ΔespF | Deletion of espF from WT EPEC | 50 |
| Δmap | Deletion of map from WT EPEC | 51 |
| ΔespH | Deletion of espH from WT EPEC | 30 |
| ΔespG1 | Deletion of espG1 from WT EPEC | This study |
| ΔespG1 ΔespG2 | Deletion of espG2 from ΔespG1 strain | This study |
| ΔespG1-FL | ΔespG1 mutant complemented with full-length espG1 | This study |
| ΔespG1 ΔespG2-FL | ΔespG1 ΔespG2 mutant complemented with full-length espG1 | This study |
| ΔespG1-FL-ΔTB | ΔespG1 mutant complemented with espG1 that lacks a putative tubulin binding domain | This study |
| ΔespG1 ΔespG2-FL-ΔTB | ΔespG1 ΔespG2 mutant complemented with espG1 that lacks a putative tubulin binding domain | This study |
| WT EPEC (GFP) | WT EPEC transformed with GFP-expressing plasmid pFPV25.1 | This study |
| ΔescN (GFP) | ΔescN mutant transformed with GFP-expressing plasmid pFPV25.1 | This study |
| ΔespG1 (GFP) | ΔespG1 mutant transformed with GFP-expressing plasmid pFPV25.1 | This study |
| ΔespG1 ΔespG2 (GFP) | ΔespG1 ΔespG2 mutant transformed with GFP-expressing plasmid pFPV25.1 | This study |
| Plasmids | ||
| pFPV25.1 | GFPmut3a is expressed constitutively, ampicillin resistant | 52 |
| pTOPO-2HA | pCR2.1-TOPO-based expression vector, ampicillin and kanamycin resistant | 53 |
| pEspG1-FL | pTOPO-espG1-FL, ampicillin and kanamycin resistant | This study |
| pEspG1-ΔTB | pTOPO-espG1-FL-ΔTB, ampicillin and kanamycin resistant | This study |
| pRE112 | Suicide vector, chloramphenicol resistant | 47 |
| pRE112-ΔespG2 | pRE112-based construct used to make the ΔespG2 deletion mutant | This study |