FIG 6.

Characterization of CD66⁺ and CD14⁺ cell proportions within Candida granuloma-like structures over time. Peripheral blood mononuclear and polymorphonuclear cells from 10 healthy subjects were infected with 32 Candida clinical isolates for up to 6 days. The granuloma-like structures were collected from coculture plates at different time points and stained with a cocktail of fluorescence-conjugated antibodies specific to CD66 neutrophils and CD14 monocytes. (A) Representative flow cytometry analysis showing a side-scatter (SSC) versus forward-scatter (FSC) plot of granulocyte (I) and monocyte (II) selection. CD66⁺ cells were gated from region I, and CD14⁺ monocytes were gated from region II. (B) The proportions of CD66⁺ cells within granuloma-like structures are expressed as percentages of the total living cell compartment. Box plots depict median, minimum, and maximum percentages of CD66⁺ cells in persistent-infection and controlled-infection subjects. (C) Scatter plot of CD66⁺ proportions according to persistent-infection and controlled-infection status. (D) The proportions of CD14⁺ cells within granuloma-like structures are expressed as a percentage of the total living cell compartment. Box plots depict median, minimum, and maximum percentages of CD14⁺ cells according to persistent-infection and controlled-infection status. (E) Scatter plot of CD14⁺ cells proportions. *, P < 0.05; **, P < 0.001; ***, P < 0.0001; ****, P < 0.00001 (by one-way ANOVA with Tukey's multiple-comparison test) (n = 320). ns, not significant.