FIG 5.

Fluorescence recovery after photobleaching (FRAP) of fluorescently labeled membrane proteins. Cells were grown to mid-log phase in the presence of arabinose with 1 mM TC and 10 mM CaCl₂ where indicated. After near-complete photobleaching with an argon laser, the fluorescence intensity was recorded within two equally sized circular regions, one in the middle of the bleached compartment A₁(t) and the other in the middle of the unbleached compartment A₂(t), for a given time. The fluorescence recovery data were normalized to the total remaining fluorescence. (A) GFP-TcpP recovery; (B) TatA recovery. The data shown are from three independent experiments and over 10 individual cells.