Fig. 4.
LL-37 enhances pMSCs immunomodulation. a pMSCs were co-cultured with PHA-activated PBMCs in different ratios and T cell proliferation determined for flow cytometry after 5 days. pMSCs inhibited T cell proliferative response in a dose-dependent manner. b The immunosuppressive capacity of pMSCs is not influenced by the pretreatment with LL-37 (1 LL37pre and 10 LL37pre). c pMSCs were co-cultured for 5 days with PHA-activated PBMCs and LL-37 was added in the first (1 LL371d and 10 LL371d) or in the third (1 LL373d and 10 LL373d) day of culture. LL-37 enhanced the anti-proliferative effects of pMSCs on T cells. d PHA-activated PBMCs were cultured for 5 days, LL-37 being added in the first (1 LL371d and 10 LL371d) or third (1 LL373d and 10 LL373d) day of culture. LL-37 increased T cell proliferation. pMSCs and PBMCs from three healthy volunteers were used in the experiments. Results are presented as mean ± SEM. *p <0.05. **p <0.01. LL-37 cathelicidin LL-3, pMSCs human placenta-derived MSCs