Table 3.

Overlapping Genes and Length of Overlap (in bp) for the Eight MRO Genomes of Blastocystis

Isolate	ST1	ST2	ST3	ST3	ST3	ST4	ST4	ST6	ST7	ST8	ST9
	NandII	Flemming	DMP/08-326	DMP/IH:478	DMP/08-1043	DMP/02-328	DMP/10-212	SSI:754	B	DMP/08-128	F5323
GenBank Accession	EF494740	KU900235	HQ909886	HQ909888	HQ909887	EF494739	KU900236	KU900237	CU914152	KU900238	KU900239
rpl2–rps19	8	8	11	11	11	8	8	8	11	11	8
rps19–rps3	–	–	–	–	–	4	4	4	–	–	4
rps3–rpl16	–	–	–	–	–	–	–	–	–	10	–
nad7–orf160a	56	56	56	55	56	56	56	56	56	56	56
orf160–nad4	–	–	–	–	–	–	–	10	–	–	10
nad4–rps4b	–	7	–	–	–	–	11	–	–	–	–
rps8–rpl6	–	–	–	–	–	–	–	8	–	–	8
rpl6–rps2	11	11	11	11	11	11	11	8	11	11	8
rps2–rpl14	–	–	–	–	–	–	–	–	–	17	–
nad2–rps10	–	10	10	10	10	10	10	10	10	10	10
rps10–nad9	23	23	8	8	8	20	20	23	26	23	20
nad9–rps11	–	–	–	–	–	–	–	1	–	–	1
nad1–nad4L	10	10	10	10	10	10	10	10	10	10	10
rps12–tRNAAsn	7	38	7	38	38	7	7	38	69	38	69
Total overlap	115	163	113	143	144	126	137	176	193	237	204

^aThe triplet and the single letter amino acid letter designation is given.

^bThe absence in some STs of an initiation codon in rps4 and the presence of in-frame stop codons in orf160 complicates the calculation of the overlap lengths involving these genes as it is unclear where the true start of translation is. Accordingly, we have annotated the start of rps4 as the first triplet after the stop codon of the preceding nad4 gene and the start of orf160 as the conserved ATG upstream of the in-frame stop codon(s); the overlap lengths involving these two genes must be viewed as tentative.