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. 2016 Dec 1;409(2):529–538. doi: 10.1007/s00216-016-0051-6

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© The Author(s) 2016

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 1 — Experiment design. Investigating the influence of various organic mobile phases on ZIC-HILIC glycopeptide enrichment efficiency and development of a simplified HILIC enrichment technique (“Drop-HILIC”). All four mobile phases [(i) acetonitrile, (ii) ethanol, (iii) methanol and (iv) isopropanol] were tested using 80% organic solvent starting conditions. Glycopeptide enrichment efficiencies were evaluated by nanoLC ESI-MS/MS using samples of increasing complexity ranging from well-defined synthetic glycopeptides spiked into a tryptic digest of BSA over individual standard glycoproteins to a tryptic digest of depleted and non-depleted human serum