Fig. 2.

Viability of oral fibroblasts after stimulation with different concentrations of toothpaste slurries from child dentifrices. Oral fibroblasts were exposed for 2 min to 25, 12, and 6 % concentrations of uncentrifuged and unfiltered toothpaste slurries from child dentifrices. Viability was measured with an MTT assay. The 25 and 12 % concentrations of uncentrifuged and unfiltered toothpaste slurries strongly reduced cell viability, almost independent of the toothpaste used. When using a 6 % concentration, the results mainly support the data of the experiments when toothpaste-conditioned medium was used