Table 3.
Repeatability and intermediate trueness and precision values at three different concentration levels (n = 6 per day, per level) to determine accuracy of the developed method in plasma samples
| Analyte | Targeted concentration | Mean ± SD | Units | Recovery ± RSD (%) | CVr (%) | CViR (%) |
|---|---|---|---|---|---|---|
| Homocysteic acid | 15.0 | 15.0 ± 0.8 | μM | 100.2 ± 2.1 | 6.2 | 7.7 |
| 100.0 | 102.2 ± 3.9 | 102.2 ± 1.6 | 6.6 | 7.2 | ||
| 200.0 | 199.6 ± 7.3 | 99.8 ± 1.5 | 6.3 | 6.8 | ||
| Taurine | 30.0 | 29.6 ± 2.8 | μM | 98.6 ± 3.8 | 12.5 | 14.7 |
| 200.0 | 188.0 ± 14.3 | 94.0 ± 3.1 | 7.3 | 10.1 | ||
| 400.0 | 370.0 ± 24.0 | 92.5 ± 4.8 | 5.7 | 8.3 | ||
| Serine | 30.0 | 30.1 ± 0.8 | μM | 100.3 ± 1.1 | 7.9 | 7.7 |
| 200.0 | 203.4 ± 6.2 | 101.7 ± 1.2 | 3.2 | 4.3 | ||
| 400.0 | 405.6 ± 11.6 | 101.4 ± 1.2 | 4.1 | 4.7 | ||
| Total cysteine | 15.0 | 16.1 ± 0.9 | μM | 107.6 ± 4.1 | 14.1 | 14.0 |
| 100.0 | 101.5 ± 1.9 | 101.5 ± 0.8 | 7.9 | 7.5 | ||
| 200.0 | 191.4 ± 12.5 | 95.7 ± 2.7 | 7.1 | 9.2 | ||
| Glycine | 30.0 | 28.6 ± 1.8 | μM | 95.3 ± 2.6 | 15.2 | 15.2 |
| 200.0 | 197.9 ± 6.1 | 99.0 ± 1.3 | 5.1 | 5.6 | ||
| 400.0 | 399.9 ± 15.8 | 100.0 ± 1.6 | 4.8 | 5.9 | ||
| Total homocysteine | 15.0 | 14.6 ± 0.4 | μM | 97.5 ± 1.0 | 2.9 | 3.7 |
| 100.0 | 100.3 ± 1.7 | 100.3 ± 0.7 | 2.2 | 2.6 | ||
| 200.0 | 197.0 ± 5.0 | 98.5 ± 1.0 | 6.2 | 6.2 | ||
| Riboflavin | 30.0 | 29.5 ± 3.1 | nM | 98.3 ± 4.3 | 18.0 | 19.5 |
| 200.0 | 193.9 ± 10.2 | 97.0 ± 2.1 | 6.2 | 7.7 | ||
| 400.0 | 399.5 ± 28.0 | 99.9 ± 2.9 | 8.6 | 10.5 | ||
| Methionine | 15.0 | 15.2 ± 0.4 | μM | 101.4 ± 1.1 | 6.1 | 6.2 |
| 100.0 | 103.1 ± 2.4 | 103.1 ± 1.8 | 2.8 | 3.5 | ||
| 200.0 | 202.7 ± 5.9 | 101.4 ± 1.2 | 3.6 | 4.4 | ||
| Pyridoxine | 15.0 | 14.4 ± 0.4 | nM | 96.0 ± 2.4 | 9.1 | 8.8 |
| 100.0 | 100.8 ± 3.2 | 100.8 ± 1.3 | 4.0 | 4.8 | ||
| 200.0 | 194.8 ± 9.3 | 97.4 ± 1.9 | 5.2 | 6.7 | ||
| Cystathionine | 30.0 | 30.8 ± 1.9 | nM | 102.5 ± 2.5 | 17.2 | 16.8 |
| 200.0 | 194.7 ± 16.8 | 97.3 ± 3.5 | 9.7 | 12.4 | ||
| 400.0 | 388.3 ± 26.5 | 97.1 ± 2.8 | 8.6 | 10.4 | ||
| SAH | 30.0 | 30.8 ± 3.1 | nM | 102.6 ± 4.1 | 17.9 | 19.1 |
| 200.0 | 202.2 ± 11.2 | 101.1 ± 2.3 | 12.7 | 12.8 | ||
| 400.0 | 398.8 ± 25.0 | 99.7 ± 2.6 | 11.7 | 12.4 | ||
| Pyridoxamine | 15.0 | 13.5 ± 1.0 | nM | 90.3 ± 6.2 | 16.0 | 16.4 |
| 100.0 | 98.5 ± 4.4 | 98.5 ± 1.8 | 7.5 | 8.2 | ||
| 200.0 | 195.1 ± 11.3 | 97.5 ± 2.4 | 9.5 | 10.4 | ||
| SAM | 30.0 | 26.5 ± 2.0 | nM | 88.4 ± 7.2 | 18.5 | 18.5 |
| 200.0 | 200.6 ± 10.0 | 100.3 ± 2.0 | 12.1 | 12.1 | ||
| 400.0 | 415.9 ± 36.7 | 104.0 ± 3.6 | 10.1 | 12.7 | ||
| DMG | 5.0 | 4.9 ± 0.1 | μM | 97.6 ± 1.4 | 5.2 | 5.1 |
| 40.0 | 39.5 ± 2.0 | 98.7 ± 2.1 | 3.9 | 6.2 | ||
| 80.0 | 79.5 ± 2.2 | 99.3 ± 1.1 | 2.4 | 3.6 | ||
| Betaine | 12.5 | 12.6 ± 0.9 | μM | 100.7 ± 2.9 | 7.0 | 9.5 |
| 100.0 | 96.3 ± 2.3 | 96.3 ± 2.1 | 4.9 | 5.1 | ||
| 200.0 | 196.3 ± 5.0 | 98.1 ± 1.0 | 3.0 | 3.7 | ||
| Choline | 5.0 | 4.9 ± 0.2 | μM | 98.8 ± 1.3 | 5.3 | 5.8 |
| 40.0 | 39.4 ± 0.8 | 98.4 ± 0.8 | 4.2 | 4.3 | ||
| 80.0 | 79.4 ± 1.7 | 99.2 ± 0.9 | 1.4 | 2.5 | ||
| 5-MTHF | 50.0 | 47.8 ± 2.7 | nM | 95.7 ± 2.3 | 15.1 | 14.9 |
| 400.0 | 393.0 ± 26.5 | 98.3 ± 2.7 | 12.8 | 13.5 | ||
| 800.0 | 804.4 ± 26.8 | 100.5 ± 1.4 | 10.5 | 10.2 |