Table 4.
Repeatability and intermediate trueness and precision values at three different concentration levels (n = 6 per day, per level) to determine accuracy of the developed method in CSF samples
| Analyte | Targeted concentration | Mean ± SD | Units | Recovery ± RSD (%) | CVr (%) | CViR (%) |
|---|---|---|---|---|---|---|
| Homocysteic acid | 12.5 | 12.9 ± 0.3 | μM | 103.2 ± 1.8 | 6.7 | 6.5 |
| 100.0 | 102.4 ± 4.2 | 102.4 ± 1.7 | 4.8 | 6.0 | ||
| 200.0 | 200.7 ± 8.5 | 100.4 ± 1.7 | 3.9 | 5.5 | ||
| Taurine | 25.0 | 25.4 ± 0.5 | μM | 101.7 ± 0.9 | 8.8 | 8.3 |
| 200.0 | 199.4 ± 10.6 | 99.7 ± 2.2 | 5.0 | 7.0 | ||
| 400.0 | 386.5 ± 16.7 | 96.6 ± 1.8 | 3.1 | 5.2 | ||
| Serine | 25.0 | 24.5 ± 1.1 | μM | 98.1 ± 1.7 | 6.5 | 7.3 |
| 200.0 | 202.6 ± 5.9 | 101.3 ± 1.2 | 3.8 | 4.6 | ||
| 400.0 | 402.8 ± 14.3 | 100.7 ± 1.4 | 2.7 | 4.3 | ||
| Total cysteine | 12.5 | 12.7 ± 0.4 | μM | 101.3 ± 1.4 | 4.7 | 5.4 |
| 100.0 | 102.8 ± 3.0 | 102.7 ± 1.2 | 3.2 | 4.2 | ||
| 200.0 | 201.0 ± 4.9 | 100.5 ± 1.0 | 2.7 | 3.5 | ||
| Glycine | 25.0 | 25.2 ± 1.3 | μM | 100.7 ± 2.1 | 5.3 | 7.0 |
| 200.0 | 201.7 ± 6.0 | 100.8 ± 1.2 | 3.4 | 4.3 | ||
| 400.0 | 393.3 ± 8.3 | 98.3 ± 0.9 | 2.5 | 3.1 | ||
| Total homocysteine | 12.5 | 12.4 ± 0.6 | μM | 99.2 ± 1.9 | 2.6 | 5.3 |
| 100.0 | 102.7 ± 1.8 | 102.7 ± 1.5 | 3.5 | 3.7 | ||
| 200.0 | 202.4 ± 4.4 | 101.2 ± 0.9 | 2.2 | 2.9 | ||
| Riboflavin | 25.0 | 25.4 ± 2.8 | nM | 101.7 ± 4.5 | 16 | 18.3 |
| 200.0 | 202.1 ± 7.8 | 101.0 ± 1.6 | 11.7 | 11.4 | ||
| 400.0 | 402.5 ± 23.8 | 100.6 ± 2.4 | 7.8 | 9.2 | ||
| Methionine | 12.5 | 12.9 ± 0.6 | μM | 103.0 ± 1.8 | 4.9 | 6.3 |
| 100.0 | 104.2 ± 4.2 | 104.2 ± 1.6 | 3.4 | 5.1 | ||
| 200.0 | 203.9 ± 6.5 | 102.0 ± 1.3 | 3.4 | 4.5 | ||
| Pyridoxine | 12.5 | 12.6 ± 0.3 | nM | 100.9 ± 0.9 | 6.6 | 6.4 |
| 100.0 | 102.3 ± 4.3 | 102.3 ± 1.7 | 3.9 | 5.5 | ||
| 200.0 | 200.7 ± 6.8 | 100.4 ± 1.4 | 3.3 | 4.5 | ||
| Cystathionine | 25.0 | 24.1 ± 1.0 | nM | 96.5 ± 1.6 | 11.4 | 11.1 |
| 200.0 | 213.9 ± 16.3 | 107.0 ± 3.1 | 7.2 | 10.1 | ||
| 400.0 | 433.1 ± 28.4 | 108.3 ± 4.7 | 7.1 | 9.2 | ||
| SAH | 50.0 | 48.1 ± 3.5 | nM | 96.3 ± 3.0 | 12.8 | 13.8 |
| 400.0 | 403.8 ± 14.3 | 100.9 ± 1.4 | 6.9 | 7.2 | ||
| 800.0 | 773.0 ± 18.5 | 96.6 ± 2.0 | 6.9 | 6.7 | ||
| Pyridoxamine | 12.5 | 12.1 ± 0.4 | nM | 96.8 ± 2.0 | 9.9 | 9.5 |
| 100.0 | 101.3 ± 3.5 | 101.3 ± 1.4 | 7.8 | 7.9 | ||
| 200.0 | 200.0 ± 8.8 | 100.0 ± 1.8 | 7.6 | 8.2 | ||
| SAM | 50.0 | 51.1 ± 4.4 | nM | 102.1 ± 3.5 | 13.9 | 15.3 |
| 400.0 | 406.2 ± 19.1 | 101.6 ± 1.9 | 8.5 | 9.1 | ||
| 800.0 | 807.2 ± 41.6 | 100.9 ± 2.1 | 5.9 | 7.5 | ||
| DMG | 5.0 | 5.0 ± 0.2 | μM | 99.8 ± 1.2 | 3.1 | 4.1 |
| 40.0 | 40.6 ± 1.1 | 101.5 ± 1.1 | 3.2 | 4.0 | ||
| 80.0 | 80.8 ± 2.1 | 101.0 ± 1.0 | 2.8 | 3.6 | ||
| Betaine | 5.0 | 5.2 ± 0.2 | μM | 102.5 ± 1.5 | 5.2 | 6.1 |
| 40.0 | 41.1 ± 1.0 | 102.8 ± 1.7 | 3.2 | 3.8 | ||
| 80.0 | 80.5 ± 2.6 | 100.7 ± 1.3 | 2.3 | 3.8 | ||
| Choline | 5.0 | 5.0 ± 0.1 | μM | 100.2 ± 1.2 | 4.9 | 5.3 |
| 40.0 | 40.7 ± 1.0 | 101.8 ± 1.0 | 3.1 | 3.8 | ||
| 80.0 | 79.9 ± 1.0 | 99.9 ± 0.5 | 2.9 | 3.0 | ||
| 5-MTHF | 50.0 | 49.5 ± 3.9 | nM | 99.1 ± 3.2 | 12.5 | 13.9 |
| 400.0 | 408.0 ± 7.7 | 102.0 ± 0.8 | 5.7 | 5.5 | ||
| 800.0 | 818.2 ± 29.8 | 102.3 ± 1.5 | 3.8 | 5.0 |