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. 2016 Dec 18;2016:6430407. doi: 10.1155/2016/6430407

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Copyright © 2016 Li-Li Shen et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Immunohistochemistry staining for Runx2. (a–i) At 7 days (a–c), 14 days (d–f), and 21 days (g–i) after surgery, different numbers of Runx2-positive cells with brown-stained nuclei could be found surrounding the trabecular bone in the newly formed bone area in the pLenti6.3-ctrl group (a, d, g), the pLenti6.3-efnb2siRNA group (b, e, h), and the pLenti6.3-ephb4siRNA group (c, f, i). (j) Quantitative analysis via IOD measurements using the Image-Pro Plus 6.0 software. Arrow, positive Runx2 staining; scale bars, 50 μm; ^∗ p < 0.05, ^∗∗ p < 0.001 versus pLenti6.3-ctrl group.