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. 2016 Dec 18;2016:3894816. doi: 10.1155/2016/3894816

Figure 2.

Figure 2

Silencing SART1 antagonizes the antiviral activity of IFN-α. (a–c) HepG2 cells were cotransfected with HBV-1.3 and Si-SART1 for 24 h. The cells were then treated with 1000 IU/mL IFN-α for 24 h. 48 h after transfection, the supernatants were collected and assayed for quantification of HBV DNA by real-time PCR, HBeAg, and HBsAg by ELISA. An siRNA-negative control was used as a control. (d) HepG2 cells were transfected with si-SART1. 48 h after transfection, the efficiency of si-SART1 was detected by qRT-PCR and western blot. Means ± SD from three independent experiments performed in triplicate are shown. Student's t-test was used to determine significance of differences between two groups. ∗ p < 0.05, and ∗∗ p < 0.01.