Figure 3.

Silencing SART1 decreases the expression of IFN-α downstream effectors. (a, b, c) HepG2 cells were transfected with si-SART1 or si-NC for 24 h and then treated with 1000 IU/mL IFN-α for 24 h. 48 h after transfection, the mRNA levels of MxA, OAS, and PKR were analyzed by qRT-PCR. ^∗ p < 0.05. (d) HepG2 cells were transfected with Si-SART1 or empty vector for 24 h and then treated with 1000 IU/mL IFN-α for 24 h. 48 h after transfection, the protein levels of MxA, OAS, and PKR were analyzed by western blotting. Means ± SD from three independent experiments performed in triplicate are shown. Student's t-test was used to determine significance of differences between two groups. ^∗ p < 0.05.