Figure 4.

Thai fruit extracts attenuated intracellular ROS production in HEK-293 cells. (a-b) Cells were treated with vehicle (control), 10 and 100 μM vitamin C, or 10 and 100 μg/ml fruit extracts for 6 h. Cells were then incubated with 200 μM H₂O₂ for 30 min. Cells were washed and incubated with 10 μM DCFH-DA. Cells were visualized by green fluorescence for DCF and by bright field. Scale bar, 10 μm (n = 4).