Fig. 4.

The double immunofluorescence staining. Neurons, astrocytes and microglia (red) expressed by TRITC. The apoptotic cell (green) shown as TUNEL positive staining (Figure 4A1, 4A2 and 4A3) in hippocampus co-localized with neurons (red) shown as NeuN positive staining (Figure 4B1). However, the apoptotic positive cells in hippocampus rarely co-localized with astrocyte (red) shown as GFAP positive staining (Figure 4B2) and also rarely co-localized with microglia (red) shown as iba1 positive staining (Figure 4B3). Image C was the merger of A and B. The merged images indicated that the apoptotic cells in the hippocampus co-localized with neurons (yellow, Figure 4C1) but rarely with astrocytes (Figure 4C2) and microglia (Figure 4C3). Scale bars indicated 50µm.