Fig. 6.

The immunofluorescent staining of the kappa opioid receptor (KOR) after MCAO. The positive staining of KOR was shown as bright green cells against to the background. After MCAO, the number of KOR increased in the ischemic cortex as indicated in Figure 6A as compared to that in the contralateral cortex (penumbra area) (Figure 6B). A high level of KOR expression was detected in the Purkinje cell layer of the cerebellum instead of molecular layer and granule cell layer (Figure 6C). In the hippocampus, the KOR expression also elevated in the ischemic side than that in the non-ischemic side (Figure 6D). Small white arrows indicate examples of the positive cells. Scale bars indicated 50µm. MCAO, middel cerebral artery occlusion.