Fig. 5.

Probe performance in glass microfluidic devices. a) Trace shows detection of changes from 0 to 100 nM fluorescein in a 25 µm deep × 80 µm wide glass microfluidic channel. b) Calibration curve of fluorescein concentrations corresponding to (a), resulting in detection limit of 11 nM (S/N =3). c) Image of the probe on a glass-based electrophoresis chip, demonstrating the feasibility of using probe as a plug and play device. d) Probe detection on the electrophoresis chip of a 0.1 mg/mL protein ladder with 15 µm deep × 50 µm wide detection channel, showing electropherograms of 7 separated proteins. Doublet feature of the first protein was due to degradation of sample and/or gel, or separation conditions. These discrepancies do not influence detection.