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. Author manuscript; available in PMC: 2018 Apr 1.
Published in final edited form as: Clin Immunol. 2016 Jul 1;177:76–86. doi: 10.1016/j.clim.2016.06.013

Figure 3. Tumor cell expression of H-2Kb following incubation with IFNγ, CTLs, A-NK cells, or medium from CTL and A-NK cell cultures.

Figure 3

A: Tumor cell expression of H-2Kb after four and sixteen hours of co-culture with OVA-specific (OT-I) CTLs and A-NK cells. Expression of MHC class-I by B16-M05 tumor cells, which had been incubated with OVA-specific (OT-I) CTLs or A-NK cells for 4–16 hours in the presence (black columns) or absence (grey columns) of blocking antibodies to IFNγ was determined by flow cytometry. MHC class-1 was visualized by staining with PE-conjugated H-2Kb antibody. Black: non-stained cells; Green: isotype control; Red: B16-M05 cells; Blue: B16-M05 cells + A-NK cells (E:T=1:1); Orange: B16-M05 cells + CTLs (OT-I). Representative of two experiments. B: H-2Kb expression by B16-M05 tumor cells was measured by flow cytometry after 16 hours of incubation with IFNγ (5–40ng/ml) or conditioned medium from mock, IL-12, IL-18, and IL-12+IL-18 gene-transduced A-NK cells (collected at 72 hours after the transduction). All incubations were performed in the presence (black columns) or absence (grey columns) of blocking antibodies to IFNγ Values represent Mean Fluorescence Intensity (MFI) Representative of two (IFNγ) and three (NK-supernatants) experiments.