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. Author manuscript; available in PMC: 2018 Apr 1.
Published in final edited form as: Clin Immunol. 2016 Jul 1;177:76–86. doi: 10.1016/j.clim.2016.06.013

Figure 5. Rapid lysis of NK cell-conditioned tumor cells by tumor-specific CTLs.

Figure 5

To determine the influence of pre-treatment with NKCM on the kinetics of tumor cell lysis by tumor-specific CTLs, B16-M05 tumor cells were incubated with 50% NKCM for 18–25 hours. NKCM pretreated and non-treated, GFP+ B16-M05 tumor cells were mixed with CellTracker Orange labeled OT-I-CTLs (E:T = 3:1) and observed by live cell microscopy for 15 hours (DIC, red and green fluorescence images were acquired every 3 minutes). A: Images of co-cultures of OT-I-CTLs and NKCM pretreated (upper row: NKCM) and non-treated (lower row: Control) B16-M05 tumor cells acquired after approximately 0 h, 5 h, 10 h and 15 h of incubation. Left panels show merged DIC and red fluorescence images. Right panels show merged red and green fluorescence images. B: The time of lysis of individual tumor cells within 0–5, 5–10 and 10–15 hours of culture was determined in 12 randomly chosen areas of each co-culture (NKCM and Control). Data are expressed as the percentage of NKCM-treated (light columns) and control (dark columns) tumor cells killed within a 5 hour period relative to all NKCM-treated and control tumor cells, respectively, killed between 0 h and 15 h. *: p<0.05. Numbers in columns indicate the percentage of tumor cells killed within a 5 hour period relative to all tumor cells that could have been killed between 0 h and 15 h. Error bars indicate SE. Representative of three individual experiments.