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Nerve conduction block occurred locally at the site of Stim.B. The thick black lines under the trace indicate the duration of different stimulations. After the high frequency Stim.B completely blocked muscle contractions, an additional stimulation (0.5 Hz) applied at the site of Stim.C distal to the site of Stim.B could still induced muscle twitches, indicating the block was not due to muscle fatigue.
