Fig. 3. ELISA characterization of the anti-Ts venom sheep antibodies, per animal. Reactivity of individual sheep serum. In G1, animals 1, 2, 3 and 4 were immunized with PBS (A). In G2, animals 5, 6, 7 and 8 were immunized with crude Ts venom (B). In G3, animals 9, 10, 11 and 12 were immunized with glutaraldehyde detoxified Ts venom (C). Serum from each animal was used at different dilutions (1/100; 1/500; 1/1,000; 1/5,000; 1/10,000 and 1/50,000). Plates were coated with 100 µL of Ts crude venom (10 µg/mL). Values are means of duplicates from two independent experiments. Anti-Ts sheep serum that was previously produced by the injection of eight doses of 500 µg of Ts venom and emulsified in Freund's adjuvant was used as a positive control (C+).