Table 4. Survival rates for in vitro neutralization assay.

Measurement of survival rates after challenge with amounts corresponding to 2 LD₅₀ of Ts crude venom pre-incubated with 200 µL of serum from each individual animal immunized with either PBS (G1, animals 1, 2, 3, and 4), crude Ts venom (G2, animals 5, 6, 7, and 8) or glutaraldehyde-detoxified Ts venom (G3, animals 9, 10, 11, and 12). As a negative control (C−), venom was preincubated with 200 µL of PBS, while as a positive control (C+), venom was preincubated with commercial horse anti-Ts. To detect possible adverse effects, one group was injected with only 200 µL of a pool of all sheep sera and no venom.