Figure 1. Effect of cholesterol depleting agents on membrane cholesterol in triple negative breast cancer cells. MDA-MB 231 and 468 cells were treated with methyl-β-cyclodextrin (MβCD), nystatin and filipin III at different concentrations (0.1–0.5 mM) for 1, 24, and 48 hours and reduction in cellular cholesterol levels were measured. Reduction of cellular cholesterol with MβCD (A, B), nystatin (C, D) and filipin III (E, F) in MDA-MB 231 and MDA-MB 468 cells, respectively. The percent reduction in the cholesterol upon the treatments was calculated with respect to total cholesterol in the untreated cells, which was taken as 100%. The results represent the mean±SD of three independent experiments.