Figure 2. Effect of cholesterol depleting agents on cytotoxicity of triple negative breast cancer cells. MDA-MB 231 and 468 cells were treated with methyl-β-cyclodextrin (MβCD), nystatin and filipin III at different concentrations (0.1–0.5 mM) for 1, 24, and 48 hours and cell proliferation in terms of cytotoxicity was measured using lactate dehydrogenase assay. Cytotoxic effect of MβCD (A, B), nystatin (C, D) and filipin III (E, F) in MDA-MB 231 and MDA-MB 468 cells, respectively. The cytotoxicity was expressed as percent control. The results represent the mean±SD of three independent experiments.