Figure 7. Effect of cholesterol supplementation on survival and metastasis of methyl-β-cyclodextrin (MβCD) treated triple negative breast cancer cells. Cells were treated with MβCD for 48 hours and supplemented with cholesterol in the form of 1 mM MβCD-cholesterol complex for 24 hours. Proliferation of MDA-MB 231 cells (A) and MDA-MB 468 cells (B) in terms of cytotoxicity was measured using lactate dehydrogenase assay. Effect of cholesterol supplementation on adhesive potential of MDA-MB 231 and MDA-MB 468 (C) cells to fibronectin-coated and vitronectin-coated plates, respectively. Effect of cholesterol supplementation on Transwell invasion of MDA-MB 231 and 468 (D) cells through Matrigel and the percentage of MDA-MB 231 (E) and MDA-MB 468 (F) cells in G0/G1, S and G2/M phases. (G) Effect of cholesterol supplementation on TUNEL positive of MDA-MB231 and 468 cells. The values are expressed as means±SD of three independent experiments.