Table 2. The condition for PCR performance of different genes in this study.

Target gene	Primary denaturation	Denaturation	Annealing	Extension	Final extension
blaSHV-1	72 ° C	72 ° C	57 ° C	93° C	95° C
	5 minutes	60 seconds	60 seconds	45 seconds	3 minutes
blaTEM-1	72 ° C	72 ° C	48 °C	93° C	95° C
	5 minutes	60 seconds	60 seconds	45 seconds	3 minutes
blaVEB-1	72 ° C	72 ° C	57 ° C	93° C	95° C
	5 minutes	60 seconds	60 seconds	45 seconds	3 minutes
blaVIM-1	72 ° C	72 ° C	55°C	93° C	95° C
	5 minutes	60 seconds	60 seconds	45 seconds	3 minutes
blaPER-1	72 ° C	72 ° C	46°C	93° C	95° C
	5 minutes	60 seconds	60 seconds	45 seconds	3 minutes
blaAMPC	72 ° C	72 ° C	57 ° C	93° C	95° C
	5 minutes	60 seconds	60 seconds	45 seconds	3 minutes
INT-1	72 ° C	72 ° C	56°C	93° C	95° C
	5 minutes	60 seconds	60 seconds	45 seconds	3 minutes
aac(3)-III	72 ° C	72 ° C	56°C	93° C	95° C
	5 minutes	60 seconds	60 seconds	45 seconds	3 minutes
aac(6')-1	72 ° C	72 ° C	55°C	93° C	95° C
	5 minutes	60 seconds	60 seconds	45 seconds	3 minutes