Figure 2.

Nox2 mRNA expression in the ischemic brain tissue after 90-minute middle cerebral artery occlusion followed by 3 or 22.5 hours of reperfusion.

Nox2 mRNA level was measured by real time quantitative reverse transcription-polymerase chain reaction. Ct values were normalized to rpl 32 and the comparative mRNA levels were determined by the 2^−ΔΔCt method. Experiments were repeated four times. *P < 0.05, vs. non-ischemic brain tissue (mean ± SEM, n = 8, paired t-test). Min: Minute; h: hour.