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. 2016 Nov;11(11):1816–1823. doi: 10.4103/1673-5374.194753

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Copyright: © Neural Regeneration Research

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.

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Western blot assay of expression of activated caspase-9 and caspase-3 as markers for apoptosis in spinal cord tissue in a SCI rat model.

(A) Western blots of activated caspase-9 and caspase-3 at 3 and 7 d in the spinal cord of MP-treated (SCI + MP), saline-treated (SCI + saline), and sham-operated (laminectomy alone) rats. (B, C) Quantification of activated caspase-9 (B) and caspase-3 (C) at 3 and 7 d post-SCI. The optical density of protein bands was normalized to the β-actin control. All data are expressed as the mean ± SD (n = 4; one-way analysis of variance and the least significant difference test). *P < 0.05, **P < 0.01, ***P < 0.001. MP: Methylprednisolone; SCI: spinal cord injury; d: days.