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. 2016 Nov;11(11):1830–1838. doi: 10.4103/1673-5374.194755

Figure 1.

Figure 1

Identification of Slp2-a as a Rab27a effector in cultured Schwann cells.

(A) Real-time PCR results show Rab27a (399 bp) and Rab27b (275 bp) mRNA expression in cultured pure Schwann cells. (B, C) Double-staining of Rab27a or Rab27b and Hoechst shows that cultured Schwann cells only express Rab27a protein. Scale bar: 20 μm. (D) RT-PCR results show mRNA levels of eleven Rab27a/b effectors in cultured Schwann cells. Eleven Rab27a/b effectors, including Slac2-b (407 bp), Slac2-c (566 bp), Slp2-a (713 bp), Slp3-a (744 bp), and Slp4 (312 bp) are readily visualized. (E) Western blotting results show high expression of only Slp2-a was in cultured Schwann cells. (F) Triple immunostaining of Slp2-a, Rab27a, and Hoechst reveals co-localization of Slp2-a with Rab27a in cultured Schwann cells. Scale bar: 10 μm. (G) Co-immunoprecipitation experiments show that Slp2-a interacts with Rab27a in cultured Schwann cells.