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. 2016 Nov;11(11):1830–1838. doi: 10.4103/1673-5374.194755

Figure 4.

Figure 4

Rab27a and Slp2-a siRNA reduces Rab27a and Slp2-a expressions in cultured Schwann cells.

(A, B) Schwann cells were transfected with (A) siRNA-Rab27a and (B) three different sequences of siRNA-Slp2-a. mRNA levels of Rab27a and Slp2-a are significantly reduced following respective siRNA transfections. (C) Quantification of Rab27a and Slp2-a mRNA levels in transfected Schwann cells was detected by western blot assay. Optical density results are presented as a fold of Gapdh expression. (D, E) Schwann cells were transfected with (D) siRNA-Rab27a and (E) siRNA-Slp2-a sequence #3. Each siRNA dramatically reduces expression of their respective target genes. (F) Quantification of Rab27a and Slp2-a protein levels in siRNA-transfected Schwann cells by western blot assay. Optical density results are presented as a fold of β-actin expression. Data are presented as the mean ± SEM. Student's t-test, n = 3 cultures/group. *P < 0.05, vs. control group.