Figure 2.

NGF inhibited the levels of ROS and MDA elevated by PA in RGC-5 cells.

(A) RGC-5 cells were pretreated with NGF (50 ng/mL), and then incubated with 100 μM PA for 12 hours (magnification × 200). The fluorescence intensity of DCFH-DA showed that NGF reduced the accumulation of ROS (arrows) caused by PA. (B) ROS levels in RGC-5 cells after exposure to PA with or without NGF pretreatment. (C) MDA levels in RGC-5 cells. ROS and MDA levels are presented as a percentage of control (n = 3, one-way analysis of variance and least significant difference post hoc test). *P < 0.05, vs. control group; #P < 0.05, vs. PA group. PA: Palmitic acid; NGF: nerve growth factor; ROS: reactive oxygen species; MDA: malondialdehyde.