Figure 4.

NGF stimulated the phosphorylation of Akt, FoxO1, and ERK1/2 in RGC-5 cells.

(A) RGC-5 cells were treated with different concentrations of NGF for 10 minutes. Levels of phosphorylated Akt, FoxO1 and ERK1/2 were analyzed by immunoblotting. (B) Relative levels of p-Akt versus total Akt, p-FoxO1 versus total FoxO1 and p-ERK1/2 versus total ERK1/2 in each sample were determined by densitometry and are expressed as a percentage of control, which was set at 100%. Results are shown as the mean ± SEM and represent three independent experiments (n = 3, one-way analysis of variance and least significant difference post hoc test). *P < 0.05, vs. control group (0 ng/mL NGF). NGF: Nerve growth factor.