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. 2016 Oct 13;66(1):193–205. doi: 10.2337/db16-0915

Figure 1.

Figure 1

Metformin reduces atherosclerotic lesions in diabetic ApoE−/− mice, but not in diabetic ApoE/AMPK-α2−/− mice. ApoE−/− and ApoE−/−/AMPK-α2−/− mice were induced with diabetes by STZ injection and treated with metformin (Met; 300 mg/kg/d) or vehicle (Veh) for 12 weeks (n = 8–10 per group). A: Representative images of Oil Red O staining of atherosclerotic lesions at the aortic sinus. B: Quantitative analysis of atherosclerotic lesion size in the aortic root. C: Representative images of Sudan IV staining of atherosclerotic lesions at the aortic arch. D: Quantitative analysis of the en face atherosclerotic lesion area in the aortic arch. *P < 0.05 vs. control mice (Con); #P < 0.05 vs. Veh. Phosphorylation of AMPK at Thr-172 (p-AMPK) (E) and expression of total AMPK-α1 and AMPK-α2 in the aorta (F) were was determined by Western blotting. n = 4; *P < 0.05 vs. Veh; #P < 0.05 vs. WT.