Figure 1.

Measurements of TF concentration-dependent expression for thousands of designed promoters. (A) Schematic depiction of the experimental design. A pooled library of 6500 designed promoters was transformed into yeast, and expression levels of all strains in the pooled library were measured in minimal media at each of six different amino acid concentrations (see Methods). (B) Promoter expression measurements sorted by dynamic range. For each promoter in the library, we obtain an expression measurement at each of the six AA concentrations. For promoters that lack Gcn4, Leu3, Bas1, or Met31 sites, expression does not change with decreasing AA concentration (top of B). For promoters with multiple Gcn4 binding sites, expression increases with decreasing [AA]. The trans-activating transcriptional activity of these transcription factors increases with decreasing [AA]. (C) Shown are four representative induction curves showing the effect of changing the number of Gcn4 binding sites (cyan, green, blue) or adding a polyT nucleosome disfavoring sequence (green, red). IDs show library construct identifiers.