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. 2016 Dec 1;30(23):2565–2570. doi: 10.1101/gad.289553.116

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© 2016 Lahmy et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 3. — Pol V lncRNAs or the act of their transcription primarily mediate the association of Pol V to chromatin. (A) Amino acid sequences of the catalytic sites (metal A) of different polymerase subunits from Arabidopsis thaliana, Saccharomyces cerevisiae, and Escherichia coli. (B) qRT–PCR analysis of Pol V lncRNA levels in Col-0 and nrpe1-11 and nrpe1-3 mutants. (Rel. Tran. Lev.) Relative transcript level. Values represent the means ± SD of three independent experiments. (C) ChIP analysis of Pol V binding in nrpe1-11 and nrpe1-3 mutants at different RdDM targets, as indicated at the right. Actin2 and Actin7 were used as controls. Values are means ± SD of two independent amplifications. After formaldehyde cross-link, Pol V complexes were immunoprecipitated using either the anti-NRPE5 or anti-NRPE1 antibody.