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. 2016 Dec 1;30(23):2596–2606. doi: 10.1101/gad.288258.116

Figure 2.

Figure 2.

sxe2 transcript rhythms in the fat body are regulated by insulin and feeding in the absence of a clock. (A) Wild-type (Iso31) flies displayed rhythmic sxe2 transcript expression in the fat body (P = 0.047) as assessed by JTK_Cycle analysis of quantitative RT–PCR (qRT–PCR) data. (B,C) Loss of DILP2 (B) or DILP2, DILP3, and DILP5 (C) resulted in loss of sxe2 transcript rhythm. P = 1.0 and P = 0.42, respectively. (D) Expression of a dominant-negative insulin receptor in the fat body (takeout-Gal4 > UAS-InRDN) resulted in a loss of sxe2 transcript rhythm. P = 0.88. (E) On the fourth day in DD, wild-type flies do not maintain rhythmic sxe2 transcript expression (filled symbols). P = 1.00. Restricted feeding (RF; open symbols) from circadian time (CT) 9–15 does not restore rhythmic sxe2 transcript expression. P = 0.11. (F) On the fourth day in DD, period mutant (per01) flies do not show rhythmic sxe2 transcript expression (filled symbols). P = 0.35. Restricted feeding (RF; open symbols) from CT 9–15 restores a circadian rhythm with a peak at CT 16. P = 2.0 × 10−4. n = 3 biological replicates per time point for A–C, E, and F. n = 2 biological replicates per time point for D.