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. 2016 Dec 1;30(23):2637–2648. doi: 10.1101/gad.287045.116

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© 2016 Kanatsu-Shinohara et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 5. — Derivation of GS cells in a B6 background by PS48. (A) ECAR of GS cells cultured with GDNF and FGF2 for 3 d. n = 14 for control; n = 15 for FGF2 + GDNF. (B) ECAR of 7-d-old DBA or B6 germ cells cultured with GDNF and FGF2. n = 12 for DBA; n = 11 for B6. (C) Germ cell tumors found in recipient testis that underwent transplantation of testis cells overexpressing Myc or Mycn. (D) Appearance of DBA-GS and PS48-B6-GS cells. (E) ECAR of 7- to 8-d-old B6 germ cells cultured with GDNF, FGF2, and PS48. n = 14 for control; n = 13 for PS48. (F) Proliferation of B6-GS cells. Bars: C,D, 50 µm. The asterisk indicates a significant difference.