Extended Data 4. Elevated [K+]e suppression of TCR induced Akt-mTOR signalling limits activation induced nutrient consumption and T cell effector lineage commitment.
(a-b) Flow cytometry analysis of the indicated phospho-proteins in CD8+ T Cells stimulated via anti-CD3 and anti-CD28 cross-linking in the indicated conditions. (c) 2-NBDG uptake in primed CD8+ T cells induced by TCR stimulation in the indicated conditions with representative histograms and quantification. (d) Seahorse XF Bioflux analysis of CD3-28 Dynabead induced extracellular acidification (ECAR) and oxygen consumption rate (OCR) of CD8+ T cells in the indicated conditions (e) Flow cytometry analysis of CD4+ T cells polarized in the indicated experimental condition concurrently with (e) TH1, (f) TH17, or (g) iTreg cytokines. Elevated [K+]e equal to 40mM. Error bars represent mean ± SEM. *P < 0.05; **P< 0.01 ***P < 0.001; ****P < 0.0001 between selected relevant comparisons, 2-way ANOVA (a,b), 2-tailed Student’s t tests (c-g).Three technical or (c-g) culture replicates per data point. (a-g) Representative of two independent experiments.