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. Author manuscript; available in PMC: 2017 Mar 14.
Published in final edited form as: Nature. 2016 Sep 14;537(7621):539–543. doi: 10.1038/nature19364

Extended Data 5. Pharmacologic inhibition and genetic disruption of PP2A function restores T cell effector function in elevated [K+]e.

Extended Data 5

(a) Flow cytometry analysis of the indicated phospho-proteins in primed CD8+ T cells stimulated via TCR-crosslinking in the indicated conditions. (b-c) Flow cytometry analysis of CD8+ T cell IFN-γ production following immobilized anti-CD3/28 and induced stimulation in the indicated conditions (b), or among cells expressing a PP2A_DN isoform (c). (d) Ppp2r2d gene expression in the indicated populations followed by flow cytometry analysis of IFN-γ production of the same. (e) Flow cytometry analysis of IFN-γ production by CD8+ T cells expressing an Akt1-CA isoform stimulated in the indicated conditions. Error bars represent mean ± SEM. *P < 0.05; **P< 0.01 ***P < 0.001; ****P < 0.0001 between selected relevant comparisons 2-way ANOVA (a), 2-tailed Student’s t tests (b-e) where noted (a-e) additional [K+]e equal to 40mM, (a-e) three culture replicates per condition, (a-e) representative of at least two independent experiments.

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