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. 2016 Dec 24;22:1468–1489.

Table 2. Cell-specific primary antibodies used for immunohistochemistry.

Primary Antigen Imunogen Source Catalog. No. Target Host Dilution
CHX10
Recombinant amino acids 1-131 N-terminal of human protein
Exalpha Biologicals
X1180P
RPCs in development
Bipolar cells in adult
Sheep
1:200
CcnD1
Recombinant fusion protein from mouse
Santa Cruz
SC-450
Proliferative RPCs
Mouse
1:200
PCNA
Purified PCNA
Chemicon
MA4076
Proliferative RPCs
Mouse
1:100
PKCα
Protein kinase C alpha
AbD Serotec
MCA1572
Rod bipolars
mouse
1:100
PMCA
Purified human erythrocyte ATPas
Affinity Bioreagents
MA3-914
OPL, IPL some amacrine and bipolar cells
Mouse
1:500
Recoverin
KLH-conjugated linear peptide corresponding to a sequence from the EF-hand 4 domain of human recoverin
Chemicon
AB5585
Photoreceptors
Rabbit
1:1500
Rhodopsin
Bovine rhodopsin C-terminus, last 9 amino acids
Chemicon
MAB5356
Rod photoreceptors
Mouse
1:5000
Secretagogin
Amino acids 186-276 C-Terminus of SCGN of human origin
Santa Cruz
SC-135395
Cone bipolars
Rabbit
1:1000
SV2B
amino acids 1-126 mapping at the rat N-terminust
Gift from Roger Janz
 
Photoreceptor and bipolar cell ribbon synapses
Rabbit
1:1000
TRPM1
Synthetic peptide of transient potential cation, subfamily M, member 1 variant delta
Osenses
OST00037W
Dendrites of ON bipolar cells
Sheep
1:300
VGluT1 Synthetic peptide from rat VGLUT1 protein Chemicon AB5905 Synaptic layers Guinea Pig 1:1000