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. Author manuscript; available in PMC: 2018 Jan 1.
Published in final edited form as: Pharmacol Res. 2016 Dec 8;115:299–308. doi: 10.1016/j.phrs.2016.12.005

Figure 3.

Figure 3

Alcohol stimulates the phosphorylation of ErbB2, FAK and cSrc. A: MCF7-ErbB2 cells were pretreated with alcohol for 48 hours and plated to fibronectin-coated coverslips, allowing attachment for 1 hour. Phosphorylation of FAK (Tyr861) and ErbB2 (Tyr1248) was detected with immunofluorescent staining. Arrows indicate the co-localization of pErbB2 and pFAK. B: MCF7-ErbB2 cells were pretreated with alcohol for 6–48 hours, and allowed to attach for 3 hours. Cell lysates were collected and analyzed for the phosphorylation of FAK and cSrc with immunoblotting. The relative levels of pFAK and pcSrc were quantified and normalized to the expression of FAK and cSrc, respectively. * denotes significant difference from respective control groups. Alcohol significantly increased the phosphorylation of ErbB2, FAK and cSrc [37].