Table 2.
Sample | Total reads | Total OTUs | Reads subsampled | Observed species | Chao1 | Shannon | PD |
---|---|---|---|---|---|---|---|
Rumen | 6,070±766 | 651±222 | 1,038 | 457.200±133.200 | 1,071.704±303.257 | 8.113±1.421 | 26.055±4.415 |
Reticulum | 4,993±419 | 633±195 | 1,038 | 454.000±119.100 | 1,057.464±299.901 | 8.065±1.077 | 24.866±3.778 |
Omasum | 12,765±1,222 | 789±353 | 1,038 | 572.100±203.300 | 1,819.647±401.110 | 8.525±1.609 | 32.331±4.994 |
Abomasum | 6,965±891 | 703±347 | 1,038 | 521.500±194.600 | 1,315.026±307.458 | 8.334±1.522 | 30.340±4.636 |
Duodenum | 2,392±432 | 362±109 | 1,038 | 326.000±79.500 | 669.954±178.903 | 7.004±0.778 | 20.650±2.330 |
Jejunum | 1,207±367 | 322±99 | 1,038 | 316.100±77.900 | 640.548±162.316 | 6.957±0.765 | 20.924±2.421 |
Ileum | 6,359±808 | 648±272 | 1,038 | 476.200±123.000 | 1,260.505±338.792 | 7.993±0.993 | 26.626±3.909 |
Cecum | 5,310±776 | 644±268 | 1,038 | 447.600±108.800 | 916.780±183.394 | 8.102±1.399 | 24.127±3.111 |
Colon | 1,768±564 | 526±190 | 1,038 | 372.000±99.100 | 721.966±137.115 | 7.585±0.820 | 20.432±2.027 |
Rectum | 2,577±367 | 615±213 | 1,038 | 445.700±105.200 | 975.539±159.533 | 7.947±0.986 | 26.944±4.383 |
Data are expressed as average±standard deviation (n = 5 sheep). The number of observed species, Chao 1 estimator, Shannon index and phylogenetic distance (PD) index were analyzed after reads subsampled to 1,038 reads. The operational taxonomic units (OTUs) were defined with 3% dissimilarity.