Table 2.
Biological functions determined in IPA for genes differentially regulated between lamb and ewe follicles
| Categories | Functions annotation | p-value | Activation z-score | Molecules | No. |
|---|---|---|---|---|---|
| Cellular growth and proliferation | Proliferation of cells | 3.88×10−10 | 3.602 | CTGF, HMMR, GCLC, MKI67, SKAP2, RECQL, PTP4A2, CAV1, JARID2, AMACR, CAPN6, IL4R, PTCH1, RRM2, GRK5, PLD1, NINJ1, OXT, PCNA, NPR3, ZBED6, CTSB, CLSPN, NOTCH1, UBE2C, NRP1, ADM, IL1RL1, MYH11, OGT, HK2, NTRK1, GRIK2, FAP, CITED2, ENPP1, ACTB, CDC6, CDK1, RRM1, MCM3, SEMA3A, NOV, SREBF1, FEN1, NEDD9, SEMA7A, LIG1, CDCP1, FGL2, HSPA5, PBK, LMNB1, GABARAPL1, PIWIL2, POSTN, CHST11, KPNA2, MYBL2, NEDD4L, FOSL2, BRCA1, IFT172, ADAMTS4, PDK4, MCM5, CLEC1B, PTX3, PAPPA, SPP1, IL6R, RGS4, HELLS, MYOF, STC1, LMNB2, SCD, ANGPT2, CDC20, GADD45G, DTYMK, MCM4, CCNA2, NFAT5, CLK1, NFKBIA, CYP19A1, THBS1, CSPG4, MANF, SLC20A1, ARID5B, CKS2, UHRF1, VWF, MT1A, BIRC5, IVNS1ABP, B4GALT1, RNF111, EFEMP1 | 101 |
| Cancer | Digestive system cancer | 2.18×10−12 | 2.804 | SNTB2, MCM6, CTGF, L3MBTL3, SYTL2, PCSK1, TFR2, HMMR, GCLC, MKI67, CHAF1A, RECQL, JARID2, CAV1, TDP2, HSD17B1, AMACR, BCOR, PTCH1, LRRC16A, GRAMD1B, RRM2, GRK5, UBE2S, PLD1, PCNA, NPR3, ST3GAL1, CTSB, ARHGEF28, FAM13C, TSPYL4, CEP95, CLSPN, LRP8, NOTCH1, NRP1, UBE2C, CHCHD10, IL1RL1, USP53, ZBED5, HYOU1, MYH11, CRYBG3, REG4, OGT, ISYNA1, AMN1, HK2, PPAP2B, NTRK1, ANTXR2, ABHD2, DSTYK, LNPEP, GRIK2, FAP, VEPH1, CITED2, ADGRB2, STAMBPL1, ENPP1, PMM1, NELL2, ACTB, FAM63A, CDC6, POLE, RRM1, MCM3, SEMA3A, SDF2L1, NOV, LDLR, GPBP1, SREBF1, MAN1A1, FEN1, INADL, NEDD9, KANSL2, WDR19, COBLL1, SEMA7A, LIG1, CDCP1, SERPINA12, KIAA1841, C3orf33, SYNE1, HSPA5, LMNB1, PBK, CHST15, SEMA6D, TMTC2, PIWIL2, LPL, AMPH, POSTN, PDIA4, CHST11, KPNA2, TUBA1C, MYBL2, CDCA3, BRCA1, CCDC64, PDK4, ADAMTS4, IFT172, PTX3, DNAH9, PHYHIPL, MCM5, NBEAL1, ATP6V1C2, PAPPA, SPP1, IL6R, MTUS2, RGS4, HELLS, FAM222B, KCNK12, ADAMTS9, MYOF, RHBDF2, RLF, JAM3, LMNB2, ELL2, C20orf194, GMPPB, ABCD1, SCD, ANGPT2, CDC20, TC2N, RAPGEF4, ALAS1, LRRK1, COL6A6, MCM4, ZNF175, GFPT2, CCNA2, SNX16, CLK1, NFAT5, CSPG4, THBS1, MANF, ASPN, ARID5B, CKS2, KLHL24, FRY, WDR73, UHRF1, NEFM, VWF, BIRC5, ATP1B3, ANGPTL5, IVNS1ABP, CRELD2, B4GALT1, RNF111, DNAJB11, EFEMP1, CEMIP | 173 |
| Cancer, organismal injury and abnormalities | Abdominal neoplasm | 3.33×10−15 | 2.671 | SNTB2, SYTL2, HMMR, PCSK1, GCLC, JARID2, CAV1, AMACR, IL4R, BCOR, RRM2, GRAMD1B, LRRC16A, PLD1, NPR3, ST3GAL1, CTSB, TSPYL4, ARHGEF28, CEP95, CXorf57, UBE2C, HSD17B10, CHCHD10, IL1RL1, ZBED5, CRYBG3, AMN1, ISYNA1, OGT, NTRK1, ANTXR2, LNPEP, FAP, PMM1, MCM3, SEMA3A, SDF2L1, LDLR, NOV, GPBP1, INADL, KANSL2, LIG1, CDCP1, FGL2, HSPA5, TMTC2, PIWIL2, LPL, PDIA4, MYBL2, TUBA1C, CCDC64, IFT172, PHYHIPL, PAPPA, MGST1, ATP6V1C2, NBEAL1, IL6R, MTUS2, HELLS, KCNK12, ADAMTS9, RLF, JAM3, LMNB2, ELL2, ABCD1, SCD, CDC20, TC2N, ALAS1, RAPGEF4, COL6A6, GFPT2, CCNA2, SNX16, NFAT5, CYP19A1, SLC20A1, FRY, BIRC5, IVNS1ABP, ANGPTL5, CRELD2, RNF111, DNAJB11, MCM6, L3MBTL3, CTGF, TFR2, MKI67, RECQL, CHAF1A, TDP2, CRIM1, HSD17B1, CAPN6, OAT, PTCH1, GRK5, UBE2S, NINJ1, PCNA, GNB2, FAM13C, LRP8, CLSPN, NOTCH1, NRP1, ADM, U SP53, HYOU1, MYH11, REG4, HK2, USP27X, PPAP2B, ABHD2, DSTYK, GRIK2, SV2C, VEPH1, ADGRB2, CITED2, ENPP1, STAMBPL1, NELL2, FAM63A, ACTB, POLE, CDC6, CDK1, RRM1, ABHD6, SREBF1, MAN1A1, FEN1, NEDD9, WDR19, SEMA7A, COBLL1, SERPINA12, KIAA1841, APOA2, C3orf33, SYNE1, PBK, LMNB1, CHST15, SEMA6D, PDE7B, AMPH, POSTN, CHST11, KPNA2, CDCA3, BRCA1, ADAMTS4, PDK4, DNAH9, MCM5, PTX3, SPP1, RGS4, TNNI3, FAM222B, MYOF, STC1, C20orf194, GMPPB, ANGPT2, SLC10A7, LRRK1, MCM4, ZNF175, CLK1, NFKBIA, THBS1, CSPG4, MANF, ASPN, FAAH, ARID5B, CKS2, WDR73, KLHL24, UHRF1, NEFM, VWF, PRKAG3, ATP1B3, B4GALT1, EFEMP1, CEMIP | 197 |
| DNA replication, recombination, and repair | DNA replication | 4.96×10−4 | 2.605 | LIG1, CCNA2, PCNA, CKS2, GADD45G, CDC6, POLE, CAV1, FEN1, BRCA1, CDK1 | 11 |
| DNA replication, recombination, and repair | Metabolism of DNA | 4.67×10−5 | 2.434 | ADM, HSD17B10, LIG1, CKS2, GADD45G, POLE, CDC6, BIRC5, CDK1, CCNA2, PCNA, NFKBIA, CAV1, CYCS, KPNA2, FEN1, BRCA1 | 17 |
| Cancer | Abdominal cancer | 2.65×10−15 | 2.376 | SNTB2, SYTL2, HMMR, PCSK1, GCLC, JARID2, CAV1, AMACR, IL4R, BCOR, RRM2, GRAMD1B, LRRC16A, PLD1, NPR3, ST3GAL1, CTSB, TSPYL4, ARHGEF28, CEP95, CXorf57, UBE2C, CHCHD10, IL1RL1, ZBED5, CRYBG3, AMN1, ISYNA1, OGT, NTRK1, ANTXR2, LNPEP, FAP, PMM1, MCM3, SEMA3A, SDF2L1, LDLR, NOV, GPBP1, INADL, KANSL2, LIG1, CDCP1, FGL2, HSPA5, TMTC2, PIWIL2, LPL, PDIA4, MYBL2, TUBA1C, CCDC64, IFT172, PHYHIPL, PAPPA, MGST1, ATP6V1C2, NBEAL1, IL6R, MTUS2, HELLS, KCNK12, ADAMTS9, RLF, JAM3, LMNB2, ELL2, ABCD1, SCD, CDC20, TC2N, ALAS1, RAPGEF4, COL6A6, GFPT2, CCNA2, SNX16, NFAT5, CYP19A1, SLC20A1, FRY, BIRC5, IVNS1ABP, ANGPTL5, CRELD2, RNF111, DNAJB11, MCM6, L3MBTL3, CTGF, TFR2, MKI67, RECQL, CHAF1A, TDP2, CRIM1, HSD17B1, CAPN6, OAT, PTCH1, GRK5, UBE2S, NINJ1, PCNA, GNB2, FAM13C, LRP8, CLSPN, NOTCH1, NRP1, ADM, USP53, HYOU1, MYH11, REG4, HK2, USP27X, PPAP2B, ABHD2, DSTYK, GRIK2, SV2C, VEPH1, ADGRB2, CITED2, ENPP1, STAMBPL1, NELL2, FAM63A, ACTB, POLE, CDC6, CDK1, RRM1, ABHD6, SREBF1, MAN1A1, FEN1, NEDD9, WDR19, SEMA7A, COBLL1, SERPINA12, KIAA1841, APOA2, C3orf33, SYNE1, PBK, LMNB1, CHST15, SEMA6D, PDE7B, AMPH, POSTN, CHST11, KPNA2, CDCA3, BRCA1, ADAMTS4, PDK4, MCM5, DNAH9, PTX3, SPP1, RGS4, TNNI3, FAM222B, MYOF, STC1, C20orf194, GMPPB, ANGPT2, SLC10A7, LRRK1, MCM4, ZNF175, CLK1, NFKBIA, THBS1, CSPG4, MANF, ASPN, FAAH, ARID5B, CKS2, WDR73, KLHL24, UHRF1, NEFM, VWF, PRKAG3, ATP1B3, B4GALT1, EFEMP1, CEMIP | 196 |
| Cellular development, cellular growth and proliferation | Proliferation of tumor cell lines | 4.72×10−8 | 2.311 | LIG1, CTGF, CDCP1, HMMR, MKI67, HSPA5, PBK, CAV1, POSTN, KPNA2, BRCA1, ADAMTS4, PDK4, AMACR, CAPN6, SPP1, IL6R, PTCH1, RRM2, PLD1, OXT, PCNA, ZBED6, CTSB, NOTCH1, UBE2C, NRP1, ADM, SCD, ANGPT2, GADD45G, CCNA2, OGT, HK2, NFKBIA, CYP19A1, THBS1, CSPG4, NTRK1, UHRF1, ACTB, MT1A, BIRC5, CDK1, RRM1, NOV, RNF111, SREBF1, FEN1, EFEMP1, NEDD9 | 51 |
| Cellular movement | Migration of cells | 5.47×10−10 | 2.276 | CTGF, CDCP1, HMMR, HSPA5, LMNB1, SKAP2, SEMA6D, PTP4A2, CAV1, POSTN, TDP2, KPNA2, BRCA1, CLEC1B, PTX3, PAPPA, IL4R, SPP1, IL6R, LRRC16A, RGS4, PLD1, NINJ1, STC1, RHBDF2, JAM3, CTSB, LMNB2, ARHGEF28, LRP8, NOTCH1, NRP1, ADM, ANGPT2, IL1RL1, HYOU1, MYH11, CCNA2, NFKBIA, NFAT5, CSPG4, CYP19A1, THBS1, PPAP2B, ABHD2, FAP, CITED2, FAAH, ARID5B, ACTB, VWF, CDK1, RRM1, MCM3, SEMA3A, ABHD6, LDLR, NOV, B4GALT1, SREBF1, CEMIP, INADL, NEDD9, ST3GAL4 | 64 |
| Cancer | Growth of pancreatic tumor | 4.19×10−5 | 2.219 | CTGF, THBS1, HMMR, CTSB, NRP1 | 5 |
| Cardiovascular system development and function | Proliferation of endothelial cell lines | 1.11×10−4 | 2.219 | ADM, PTX3, THBS1, RGS4, MYH11, PLD1, CDK1 | 7 |
| Skeletal and muscular system development and function | Thickness of bone | 1.26×10−4 | 2.219 | STC1, CTGF, SPP1, CAV1, BRCA1, LRRK1, PDK4 | 7 |
| Cellular assembly and organization | Association of chromosome components | 1.35×10−5 | 2.2 | CDC6, HELLS, BRCA1, BIRC5, MCM4 | 5 |
| Cancer | Growth of tumor | 1.98×10−7 | 2.176 | ADM, ANGPT2, CTGF, IL1RL1, HMMR, HYOU1, MKI67, HSPA5, OGT, NFKBIA, CYP19A1, THBS1, CAV1, POSTN, MYBL2, BRCA1, FAP, IL4R, SPP1, IL6R, PTCH1, RRM2, BIRC5, PLD1, NOV, RNF111, JAM3, CTSB, NEDD9, NOTCH1, UBE2C, NRP1 | 32 |
| Cell death and survival | Apoptosis of pancreatic cancer cell lines | 6.32×10−5 | 2.158 | OGT, NFKBIA, SREBF1, ZBED6, HMMR, TDP2, EFEMP1, NOTCH1 | 8 |
| Carbohydrate metabolism | Metabolism of carbohydrate | 1.58×10−5 | 2.011 | GMPPB, SCD, CTGF, ENPP1, SPP1, PMM1, APOA2, RGS4, GCLC, PRKAG3, GRK5, CHST15, PLD1, GFPT2, OXT, HK2, B4GALT1, SREBF1, NTRK1, LPL, CTSB, ASPN, CHST11, CEMIP | 24 |
| Cancer | Gastrointestinal tract cancer | 3.32×10−12 | 2 | SNTB2, L3MBTL3, CTGF, SYTL2, PCSK1, TFR2, HMMR, MKI67, CHAF1A, RECQL, JARID2, CAV1, TDP2, HSD17B1, AMACR, BCOR, PTCH1, RRM2, GRK5, UBE2S, PLD1, PCNA, NPR3, ST3GAL1, CTSB, FAM13C, CEP95, CLSPN, LRP8, NOTCH1, UBE2C, NRP1, IL1RL1, USP53, MYH11, I SYNA1, OGT, HK2, PPAP2B, NTRK1, ANTXR2, ABHD2, DSTYK, LNPEP, GRIK2, VEPH1, CITED2, ADGRB2, ENPP1, STAMBPL1, PMM1, CDC6, POLE, RRM1, MCM3, SEMA3A, SDF2L1, LDLR, NOV, GPBP1, FEN1, I NADL, NEDD9, COBLL1, SEMA7A, LIG1, SERPINA12, CDCP1, KIAA1841, SYNE1, LMNB1, PBK, CHST15, SEMA6D, TMTC2, LPL, AMPH, POSTN, CHST11, KPNA2, MYBL2, TUBA1C, BRCA1, PDK4, ADAMTS4, IFT172, DNAH9, PHYHIPL, MCM5, PTX3, NBEAL1, ATP6V1C2, PAPPA, SPP1, IL6R, MTUS2, RGS4, HELLS, ADAMTS9, MYOF, RHBDF2, JAM3, ELL2, GMPPB, ANGPT2, CDC20, TC2N, ALAS1, LRRK1, COL6A6, MCM4, GFPT2, CCNA2, SNX16, CSPG4, THBS1, ASPN, ARID5B, WDR73, FRY, KLHL24, UHRF1, NEFM, VWF, BIRC5, IVNS1ABP, ANGPTL5, CRELD2, B4GALT1, RNF111, DNAJB11, EFEMP1, CEMIP | 133 |
| Skeletal and muscular system development and function | Thickness of trabecular bone | 6.06×10−4 | 2 | CTGF, SPP1, CAV1, LRRK1, PDK4 | 5 |
The predicted activation state is inferred from the bias-corrected z-score (+, increased; −, decreased). The bias-corrected z-score is computed based on the proportion of target genes present in the dataset which are directionally regulated as expected according to known associations with functions compiled from the literature. The p value of overlap measures the statistical significance of overlap using Fisher’s exact t-test, between genes from the dataset and those known to be associated with a function.