Table 4.
Upstream regulators predicted to be inhibited in lamb follicles compared with ewe follicles, using the 2-fold differentially-regulated dataset with q<0.005, on the basis of known interactions compiled in the ingenuity pathway analysis upstream regulator analysis
| Upstream regulator | Molecule type | Activation z-score | p-value of overlap | Target molecules in dataset |
|---|---|---|---|---|
| TP53 | Transcription regulator | −3.877 | 8.02×10−12 | ACTB, BIRC5, BRCA1, CAV1, CCNA2, CDC20, CDC6, CDK1, CITED2, CTGF, CTSB, CYP19A1, FEN1, GADD45G, HK2, HMMR, IL4R, KPNA2, MCM3, MCM4, MCM5, MCM6, MKI67, MYBL2, MYOF, NFKBIA, NINJ1, NOTCH1, NRP1, OAT, PBK, PCNA, PMM1, POSTN, PTCH1, RRM1, RRM2, SPP1, SREBF1, TDP2, THBS1, UBE2C, UHRF1, ZNF175 |
| let-7 | Microrna | −3.450 | 1.35×10−9 | BRCA1, CCNA2, CDC20, CDC6, CDCA3, CDK1, MCM3, MCM4, MCM5, MCM6, RRM1, RRM2, THBS1 |
| SMARCB1 | Transcription regulator | −2.795 | 7.79×10−6 | AMPH, CA5A, CCNA2, CDC6, CDK1, MCM3, MCM4, MCM5, MYBL2, POSTN |
| RB1 | Transcription regulator | −2.785 | 2.56×10−14 | ACTB, ANGPT2, BIRC5, BRCA1, CCNA2, CDC6, CDK1, CHAF1A, CITED2, CTGF, FEN1, LIG1, MCM3, MCM4, MCM5, MCM6, MYBL2, PCNA, PTX3, RRM1, RRM2, SNTB2 |
| SPARC | Other | −2.570 | 1.23×10−4 | BRCA1, CHAF1A, LRP8, MCM6, NOTCH1, POLE, RRM1 |
| RBL1 | Transcription regulator | −2.533 | 6.58×10−12 | CCNA2, CDC6, CDK1, MCM3, MCM5, MCM6, MYBL2, NOTCH1, PCNA, RRM1, RRM2, THBS1 |
| PTEN | Phosphatase | −2.464 | 1.77×10−8 | ADM, ANGPT2, BIRC5, CDC20, CITED2, CKS2, CTSB, FAM134C, IL4R, KLHL24, MKI67, MYBL2, NTRK1, PTP4A2, RRM1, SCD, SLC20A1, SPP1, SREBF1, THBS1 |
| DYRK1A | Kinase | −2.438 | 4.81×10−8 | CDC6, CDK1, MCM3, MCM4, MYBL2, UBE2C |
| CDKN2A | Transcription regulator | −2.436 | 2.17×10−9 | BIRC5, CCNA2, CDK1, CHAF1A, CITED2, CTGF, FEN1, GADD45G, MCM4, MCM5, MKI67, MYBL2, PCNA, PTX3, RRM1, RRM2, SNTB2 |
| Rb | Group | −2.425 | 6.69×10−7 | BIRC5, CCNA2, CDC6, CDK1, MCM3, MCM5, MYBL2, PCNA |
| CDKN1A | Kinase | −2.398 | 6.96×10−16 | ACTB, BIRC5, BRCA1, CCNA2, CDC20, CDC6, CDK1, CTGF, CTSB, HSPA5, MCM3, MCM4, MCM6, MKI67, MYBL2, PBK, PCNA, RRM1, UBE2C, UBE2S, UHRF1, ZBED5 |
| dinoprost | Chemical - endogenous mammalian | −2.273 | 4.95×10−9 | ADM, CYP19A1, FOSL2, LPL, MGST1, PDIA4, PTX3, SPP1, STAR, THBS1 |
| miR-24-3p | Mature microrna | −2.236 | 2.95×10−6 | BRCA1, CCNA2, CDK1, FEN1, NOTCH1 |
| Irgm1 | Other | −2.236 | 7.15×10−5 | CCNA2, CDCA3, MKI67, RRM2, UBE2C |
| HDAC2 | Transcription regulator | −2.236 | 1.41×10−3 | BRCA1, GABARAPL1, LIG1, MCM3, MCM5, NFKBIA |
| SIM1 | Transcription regulator | −2.219 | 6.49×10−2 | ANGPT2, ELL2, IL6R, OXT, POSTN |
| TGFBR2 | Kinase | −2.213 | 1.16×10−2 | BIRC5, CTGF, MYOF, NRP1, SPP1, THBS1 |
| PD98059 | Chemical - kinase inhibitor | −2.191 | 1.57×10−9 | ANGPT2, BIRC5, CAV1, CCNA2, CTGF, CTSB, CYCS, DHRS3, HSPA5, HYOU1, LDLR, LPL, MKI67, MYBL2, MYH11, NINJ1, PCNA, POSTN, RRM2, SPP1, SREBF1, ST3GAL4, STAR, THBS1 |
| INSIG1 | Other | −2.180 | 3.82×10−3 | LDLR, LPL, PLD1, SCD, SREBF1 |
| TNF | Cytokine | −2.035 | 1.17×10−11 | ACTB, ADAMTS4, ADM, ANGPT2, B4GALT1, BIRC5, CAV1, CDO1, CHST11, CITED2, CTGF, CTSB, CYP19A1, DHRS3, FOSL2, GADD45G, GCLC, GFPT2, HK2, IL1RL1, IL4R, JARID2, LDLR, LPL, MCM3, MGST1, MT1A, NEDD9, NEFM, NFKBIA, NINJ1, NOTCH1, NOV, NRP1, PAPPA, PDIA4, POSTN, PTX3, RGS4, RRM1, RRM2, SCD, SLC20A1, SPP1, SREBF1, STAR, THBS1 |
| E2F6 | Transcription regulator | −2.000 | 2.72×10−6 | BRCA1, CDC6, LIG1, MCM3, MCM5, RECQL, RRM2 |
| TNFSF13 | Cytokine | −2.000 | 2.58×10−5 | CDC6, MCM4, MCM5, MCM6, PCNA |
| ARNT2 | Transcription regulator | −2.000 | 1.57×10−1 | ANGPT2, ELL2, IL6R, POSTN |