Table 5.

Primers used for validation of selected RNA-seq based genes by real-time reverse transcription-polymerase chain reaction analysis

Gene name	Primer sequence	Genebank NO.	RNA seq	qPCR
CYP19A1	TCGTCCTGGTCACCCTTCTG CGGTCTCTGGTCTCGTCTGG	NM_001123000.1	1.34	1.08
STAR	TCGCGGCTCTCTCCTAGGTTC ATCACTTTACTCAGCACCTCGT	NM_001009243.1	1.56	0.68
EFEMP1	AACTTCCTCAATCCATCGTCT GACTTCACCAGCACAAGCA	XM_012130548.1	−4.60	−4.44
SPP1	GAAACCCCTGATGACTCTGACC GCCATCATTTGTGCTTTCCGTA	NM_001009224.1	−3.27	−3.98
PTX3	CTGTGATTCTGTTTTGTGCTCT TCGGAGTTCTCACGACTGC	XM_004003220.2	−1.68	−1.03
NRP1	TGTGGAAAGATCGCCCCT ACTTTATTACCCCACTAGGCAT	XM_012188254.1	−2.39	−1.84
ADM	ATGAAGCTGGTTCCCGTC ACTTAGCGCCCATTTATTCCA	XM_004016162.2	−1.98	−1.76
OXT	CCTCCTGGCGTTGACCTCC GAAGCAGCCCAGCTCGTCCC	NM_001009801.2	−5.25	−1.23
GAPDH	TACTGGCAAAGTGGACATCGTT TTGATGACGAGCTTCCCGTTC	NM_001190390.1